Journal: Journal of Hard Tissue Biology

Article Title: Enhancement of Osteoblast Proliferation via ERK Signaling When Cultured on Electrically Polarized Hydroxyapatite

doi: 10.2485/jhtb.34.93

Figure Lengend Snippet: Figure 3. Time course of electret-induced p130Cas signaling. (A) Repre- sentative western blots of phospho-p130Cas, total p130Cas and GAPDH. (B) The graph shows increased p130Cas phosphorylation at residue Y410 in electret-stimulated cells (P-surface, white portion and N-surface, gray portion) compared with control cells (H-surface, black portion). *P < 0.05 vs. H-surface; data are the mean and standard deviation (n = 4).

Article Snippet: Rabbit polyclonal phospho-p130Cas, phospho-ERK, phospho-p38 07[093-098]Takaaki Masutani_kk.indd 94 2025/04/02 9:10:12 95 MAPK, JNK antibodies, and rabbit monoclonal p130Cas, ERK, p38 MAPK, phospho-JNK antibodies were from Cell Signaling Technology (Denver, USA).

Techniques: Western Blot, Phospho-proteomics, Residue, Control, Standard Deviation

Journal: Archives of Toxicology

Article Title: Thallium reabsorption via NKCC2 causes severe acute kidney injury with outer medulla-specific calcium crystal casts in rats

doi: 10.1007/s00204-024-03868-2

Figure Lengend Snippet: Downregulated expression of kidney transporter and channel genes and Ca 2+ -ATPase 5 days after Tl administration at 30 mg/kg. Quantitative real-time PCR analysis of genes encoding mTAL-related kidney transporters and channels ( a ). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Kir 1.1, potassium inwardly rectifying channel subfamily J member 1; Kir 4.1, potassium inwardly rectifying channel subfamily J member 10; NaK-ATP, Na + /K + transporting ATPase alpha 1 subunit; NKCC2, Na–K–Cl cotransporter 2. GAPDH levels served as an internal control. * P < 0.05; ** P < 0.01; *** P < 0.001. Immunohistochemistry of Ca 2+ -ATPase in the outer and inner medullae of rats in the control and Tl-loaded groups ( b ). Ca 2+ -ATPase expression is decreased in the mTAL obstructed by calcium deposits (*)

Article Snippet: The sections were incubated with a 1:100 dilution of rabbit polyclonal anti-Ca 2+ -ATPase antibody (Cosmo Bio Co. Ltd., Tokyo, Japan), followed by a 1:400 dilution of horseradish peroxidase (HRP)-labeled anti-rabbit Ig antibody (Dako, Glostrup, Denmark).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Immunohistochemistry